Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants
نویسندگان
چکیده
منابع مشابه
Firefly luciferase complementation imaging assay for protein-protein interactions in plants.
The development of sensitive and versatile techniques to detect protein-protein interactions in vivo is important for understanding protein functions. The previously described techniques, fluorescence resonance energy transfer and bimolecular fluorescence complementation, which are used widely for protein-protein interaction studies in plants, require extensive instrumentation. To facilitate pr...
متن کاملBreakthrough Technologies Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants1[C][W][OA]
The development of sensitive and versatile techniques to detect protein-protein interactions in vivo is important for understanding protein functions. The previously described techniques, fluorescence resonance energy transfer and bimolecular fluorescence complementation, which are used widely for protein-protein interaction studies in plants, require extensive instrumentation. To facilitate pr...
متن کاملFirefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants1[C][W][OA]
The development of sensitive and versatile techniques to detect protein-protein interactions in vivo is important for understanding protein functions. The previously described techniques, fluorescence resonance energy transfer and bimolecular fluorescence complementation, which are used widely for protein-protein interaction studies in plants, require extensive instrumentation. To facilitate pr...
متن کاملKinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals.
Signaling pathways regulating proliferation, differentiation, and apoptosis are commonly mediated through protein-protein interactions as well as reversible phosphorylation of proteins. To facilitate the study of regulated protein-protein interactions in cells and living animals, we optimized firefly luciferase protein fragment complementation by screening incremental truncation libraries of N-...
متن کاملApplication of a split luciferase complementation assay for the detection of viral protein-protein interactions.
Intraviral protein-protein interactions are critical for virus survival in the host. Discovery of such interactions is important to understand molecular mechanisms of viral replication and pathogenesis. The development of a cell-based assay that can be employed to examine systematically viral protein interactions is described. The method, known as the split luciferase complementation assay (SLC...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2007
ISSN: 1532-2548
DOI: 10.1104/pp.107.111740